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3/4/2010 3:35:53 PM
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Crazy4Cells
Posts 9
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Does anyone have any suggestions on how to make alginate more confocal microscopy friendly? I'm currently using a 1% (w/v) alginate gel and I am unable to image completely through the gel (~1 mm thick).
Thanks!
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6/6/2010 4:39:26 PM
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QGel
Posts 12
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Sometimes alginate is rather difficult to image because of its physical properties, but perhaps 1mm is a bit too thick. Also check the cell density - if it is too dense it could play a role.
You may wish to consider using QGel, a new hydrogel that results in excellent confocal images. Have a look at a confocal image of fibroblasts in QGel HERE
edited by QGel on 6/7/2010
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info@qgelbio.com
www.qgelbio.com
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8/20/2010 3:24:27 AM
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QGel
Posts 12
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If you want to learn how to label cells in QGel with fluorescent markers, and visualize cells in QGel using fluorescence microscopy have a look at the protocols on the QGel Support Page
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info@qgelbio.com
www.qgelbio.com
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