|
7/29/2010 9:37:42 AM
|
topic:
U Durham: Apps accepted year round
science.editor
Posts 41
|
The potential of novel 3D matrices in the formation of cephalic skeletal tissues in vitro
KEY CONTENT: Key aspects of skeletogenesis have been demonstrated to occur in cranial neural crest (CNC) explants in 2D culture via the in vivo differentiation sequence. The mechanisms creating the characteristic 3D structure of the skull in vivo are also relatively well understood. The aim is to use existing knowledge to develop a 3D culture system employing a new scaffold technology supporting the production of normally organised components of the cranial skeleton. This proof of concept opens up the prospect of a wide range of potential applications in which the correct 3D organisation of cells is the key to success ...
Funding Notes: This position is open to self-funded students only.
School School of Biological and Biomedical Sciences, Durham University
Project Supervisor(s) Dr P N Hunt, Prof S A Przyborski
Original source: http://www.findaphd.com/search/showproject.asp?projectid=21119&theorder=1&location=&univ=&disc=allsci&searchtype=b&keyword=neuroscience&scip=201&scif=0&socp=44&socd=1&socf=1&pd=0&page=3
|
|
7/14/2010 8:48:16 AM
|
topic:
TEXTBOOK: 3D Cell Culture: Methods and Protocols
science.editor
Posts 41
|
Series: Methods in Molecular Biology, Vol. 695
Haycock, John W. (Ed.)
1st Edition., 2011, 330 p. 196 illus., 98 in color., Hardcover
ISBN: 978-1-60761-983-3
Not yet published. Available: December 4, 2010
Developed for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Protocols, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose ...
Original Source: http://www.springer.com/life+sciences/cell+biology/book/978-1-60761-983-3
|
|
7/14/2010 8:11:13 AM
|
topic:
U Nottingham: closes 30 July 2010
science.editor
Posts 41
|
Repeat dose toxicity testing using nanoparticle sensors and tissue engineered scaffolds - Collaborative project between University of Nottingham and AstraZeneca. This studentship is for 4 years and is open to UK and EU citizens and will pay tuition fees and a full stipend (£13,620 for 2010/11)
KEY CONTENT:
Supervisors: Jonathan Aylott, Felicity Rose, Amir Ghaemmaghami, Claire Sadler
This exciting AstraZeneca sponsored PhD project seeks to develop a 3D immuno-competent lung model with the capability to non-invasively monitor changes in the cellular microenvironment upon stimulation with drug candidates. To achieve this, nanoparticle sensors will be incorporated into electrospun polymer scaffolds, upon which cells will be cultured. By stacking layers of polymer scaffold containing epithelial, dendritic and fibroblast cells a 3D construct will be engineered that will have self-reporting capability due to the incorporated nanoparticle sensors. This in-vitro model will allow for the collection of considerable quantitative and qualitative data on cell activation, microenvironment and cell-cell interaction in response to various stimuli without disturbing the architecture of the model, hence leaving crucial 3D cell interdependency intact and enabling repeat dose toxicity testing.
This multi-disciplinary project will include training in 3D cell culture, polymer scaffold production, nanosensor technology and fluorescence imaging techniques. It would be suitable for candidates from a wide range of scientific backgrounds with the desire to expand their expertise into new fields. The successful candidate will work within a vibrant inter-disciplinary research grouping including nanotechnology, biosensors, tissue engineering, immunology and cell biology.
For further information please email with CV to Jon.Aylott@nottingham.ac.uk.
Original Source: http://www.findaphd.com/search/showproject.asp?projectid=29000&searchtype=n&page=2
|
|
7/2/2010 12:37:07 PM
|
topic:
CPW: 3D microchannel co-culture: method and...
3dcc blogger
Posts 12
|
This week's Cool Paper of the Week is "3D microchannel co-culture: method and biological validation". This paper describes and validates the importance of a dynamic 3D co-culture model and its potential uses in personalized medicine.
Check it out!
http://www.3dcellculture.com/Search_Results/id/9160
|
|
7/2/2010 11:08:24 AM
|
topic:
Durham U: CLOSED
science.editor
Posts 41
|
School of Biological and Biomedical Sciences, Durham University
Project Supervisor(s): Prof S A Przyborski
Funding Availability: Directly Funded Project (UK Students Only)
KEY CONTENT:
Development of materials to enhance the growth and function of hepatocytes in 3D
The project is part of ongoing work on developing scaffolds for 3D cell culture, where the 3D environment encourages cells to grow in a more in vivo like manner. In particular, this project involves preparing polymeric scaffolds that are designed for the culture of liver cells in 3D. Liver cell cultures are used by the pharmaceutical industry for toxicity testing of new drug candidates; we have shown that 3D cultures produce cells that are more capable of resisting challenge with toxic agents. There are also broader applications of the research in regenerative medicine. The project will involve preparing porous polymer scaffolds, characterising the scaffolds with a variety of techniques then using them in the culture of liver cells.
Original Source: http://www.jobs.ac.uk/job/ABI850/phd-studentship/
edited by science.editor on 7/29/2010
|
|
6/29/2010 8:49:13 AM
|
topic:
JHU: Studying cells in 3-D - new cancer targets
science.editor
Posts 41
|
Another take: "3D Data Key to Next-Gen Bio Research"
KEY CONTENT:
Recent research by biomedical engineers at Johns Hopkins, published in the June issue of Nature Cell Biology, however, suggests that such 2D techniques may be giving a false picture—leading to misinterpretation of or failure to identify cell migration mechanisms that work differently in vivo than on flat substrates.
....
What they discovered was surprising: On 2D substrates, focal adhesions form easily and may last for several minutes. But, says Fraley, the shape of cells in 2D and the importance of focal adhesions limiting movement are "merely artifacts of their environment." In 3D matrices, says Wirtz, the same cells assume a distinctly different shape, "focal adhesions disappear and the role of adhesion proteins in regulating cell motility becomes different." The researchers discovered that cells moving in 3D environments make only very brief and short-lived contacts with collagen fibers surrounding them. Such loss of adhesion and enhanced cell movement are hallmarks of the metastatic process by which cancers spread throughout the body.
Original source: http://www.smartertechnology.com/c/a/Technology-For-Change/3D-Dataviz-Key-to-NextGen-Bio-Research/
|
|
6/23/2010 10:57:24 AM
|
topic:
JHU: Studying cells in 3-D - new cancer targets
science.editor
Posts 41
|
This John Hopkins article has been picked up and republished by several science media outlets. The YouTube video is linked in the Miscellaneous thread here ...
KEY CONTENT:
“Our study demonstrates for the first time that the way cells move inside a three-dimensional environment, such as the human body, is fundamentally different from the behavior we’ve seen in conventional flat lab dishes. It’s both qualitatively and quantitatively different.”
One implication of this discovery is that the results produced by a common high-speed method of screening drugs to prevent cell migration on flat substrates are, at best, misleading, said Wirtz, who is the Theophilus H. Smoot Professor of Chemical and Biomolecular Engineering at Johns Hopkins. This is important because cell movement is related to the spread of cancer, Wirtz said. “Our study identified possible targets to dramatically slow down cell invasion in a three-dimensional matrix.”
Original Source: http://gazette.jhu.edu/2010/06/21/studying-cells-in-3-d-could-reveal-new-cancer-targets/
edited by science.editor on 6/29/2010
|
|
6/23/2010 10:12:08 AM
|
topic:
YouTube: This is pretty cool.
science.editor
Posts 41
|
Cancer cells pulling on 3D collagen matrix
http://www.youtube.com/watch?v=tHttgByAYRA
This video was a supplement to this 3DCellCulture.com paper: http://www.3dcellculture.com/Search_Results/id/9150
edited by science.editor on 6/29/2010
|
|
6/23/2010 8:49:14 AM
|
topic:
St. George's U: CLOSED
science.editor
Posts 41
|
Research Assistant, Division of Basic Medical Sciences
Full-time, three years fixed-term
KEY CONTENT:
The role will be to establish murine and human 3D cell cultures for studying the interaction of Salmonella with host cells using high-throughput technologies. The 3D cultures will be challenged with a range of mutant Salmonella Typhimurium strains before collection of cells and supernatants at pre-defined intervals. RNA will be extracted and analysed using commercial microarrays; samples will also be prepared for phosphoproteome analysis. The post-holder must have significant proven capability in cell culture; prior skills in RNA handling, molecular biology and bioinformatic analysis of complex data sets will be advantageous.
Original source: http://jobs.sgul.ac.uk/Vacancy.aspx?ref=103-10
edited by science.editor on 7/2/2010
|
|
6/21/2010 10:22:44 AM
|
topic:
JCB Interview with Joan Brugge
science.editor
Posts 41
|
Journal of Cell Biology, 14 June, 189 (6): 922
Key Content: "We've been working on understanding the processes that drive these different phenotypic histologies, using a breast cancer model system we adapted from Mina Bissell's 3D cell culture work. Most recently, we've been studying the mechanisms that are responsible for the ability of tumor cells to survive without attachment to the extracellular matrix. Our 3D model system had shown us that acini start out as a solid sphere of cells. The hollow lumen is generated during morphogenesis through selective apoptosis of the inner cells. But we noticed that if you blocked apoptosis in these cells, they still died."
Original Source: http://jcb.rupress.org/
|
|
6/18/2010 1:40:15 PM
|
topic:
CPW: A strategy for fabrication of a 3D Tissue...
3dcc blogger
Posts 12
|
This week I chose "A strategy for fabrication of a three-dimensional tissue construct containing uniformly distributed embryoid body-derived cells as a cardiac patch" for Cool Paper of the Week!
This group used a series of really neat techniques to create mouse embryonic cell sheets. These researchers first cultured the cells on a hydrogel, then removed the cell sheet layers and "sandwiched" the cell sheets between layers of bovine acellular tissue to create a 3D tissue construct. Check out this paper for more details!
http://www.3dcellculture.com/Search_Results/id/9134
|
|
6/11/2010 1:00:33 PM
|
topic:
Bristol U: 2 Postdocs CLOSED
science.editor
Posts 41
|
Key Content: Two three-year postdoctoral positions are available from October 2010 in Prof Dek Woolfson’s laboratory. The project is to design, produce and develop peptides that self-assemble into alpha-helical hydrogels, and apply these in 3D cell culture. The work builds on the group’s success in designing peptide-based biomaterials (Nature Materials 8:596 (2009), and aims to move this onto real-life applications in tissue engineering and towards the clinic ....
Original Source: http://www.youngbrigades.com/scholarships-and-higher-education/uk2-postdoctoral-position-in-synthetic-biology-and-tissue-engineering.html
Nature Jobs: http://www.nature.com/naturejobs/science/jobs/147652-Postdoctoral-Research-Assistants-in-Synthetic-Biology-and-Tissue-Engineering-two-posts-reference-15501
edited by science.editor on 6/21/2010
edited by science.editor on 7/14/2010
|
|
6/9/2010 4:09:36 PM
|
topic:
Test QGel's new 3D synthetic hydrogel
QGel
Posts 8
|
Start a project and collaborate with QGel and get free samples of our matrix to grow cells in 3D. QGel has opened a call for projects.
The process is simple:
1- You submit a brief description of your project online
2- QGel experts meet weekly to evaluate project submissions
3- You are notified of project acceptance and your sample material is shipped
note. There are a limited number of vials QGel has allocated for these projects. Be sure to submit your project soon.
To find out more information on how to submit your 3D Cell Culture project using QGel, visit the website Collaborate with QGel.
|
|
6/9/2010 7:35:40 AM
|
topic:
TEXTBOOK: 3D Cell-Based Biosensors in Drug Discov
science.editor
Posts 41
|
3D Cell-Based Biosensors in Drug Discovery Programs: Microtissue Engineering for High Throughput Screening
William S. Kisaalita, University of Georgia, Athens, USA
Price: $129.95
Cat. #: 73494
ISBN: 9781420073492
ISBN 10: 1420073494
Publication Date: June 23, 2010
Number of Pages: 404
Key content:
* Provides a deep comparative analysis of 2D and 3D cultures
* Uses data from peer-reviewed publications to conclusively provide the justification for use of 3D cell cultures in cell-based biosensors (assays) for high throughput screening
* Includes difficult-to-find information such as patents, cell lines or types commonly used in drug discovery programs, and their origins
* Addresses research needs mainly in areas of detection and scaffold engineering
* Describes microenvironment factors for creating phenotypes that resemble their in vivo counterparts
* Incorporates case studies in support of 3D cell-based biosensor adoption in drug discovery programs
Original Source: http://www.crcpress.com/product/isbn/9781420073492
|
|
6/8/2010 9:46:11 AM
|
topic:
The Scientist.com: 3D Science
science.editor
Posts 41
|
Volume 24 | Issue 6 | Page 27
Date: 2010-06-01
KEY CONTENT: The role of dimensionality is a growing field in cell biology, but many labs cannot afford new tools that let cells grow in three dimensions—which could, in theory, better represent what happens in vivo. As a member of F1000’s Faculty of Cell Biology, Ken Yamada at the National Institute of Dental and Craniofacial Research flagged a recent PNAS study that described an easy alternative for scientists who want to explore 3D cell culture: paper (106:18457–62, 2009).
TS: Cells are normally grown in a single layer on a plastic surface or in suspension. What’s wrong with this approach?
KY: It’s obviously spatially artificial. You lose normal three-dimensional architecture and the normal interactions of cells with their surroundings, both with the matrix and other cells. The result is usually altered cell shape and gene expression. It seems that dimensionality by itself affects cell behavior.
The other thing about cells grown on a plastic surface is that there’s a major difference in stiffness. The substrate cannot be deformed, whereas in vivo you have elasticity of the matrix.
See article for remainder ....
Original Source: http://www.the-scientist.com/article/display/57462/
|
|
6/7/2010 8:59:41 AM
|
topic:
Kollodis BioSciences Launches 3D Hydrogel Products
Kollodis BioSciences
Posts 1
|
Kollodis BioSciences Announces Launch of MAP-based (Mussel Adhesive Protein) MAPTrix HyGelTM Line of Products: Chemically Defined Hydrogel Products for 3D Cell Culture Applications
(MALDEN, MA) June 3, 2010 - Kollodis BioSciences, Inc. has announced the launch of its novel MAPTrix HyGelTM line of products. MAPTrix HyGelTM is a chemically well-defined product based on Kollodis BioSciences' proprietary recombinant Mussel Adhesive Protein technology. MAPTrix HyGelTM is an in situ formable hydrogel product designed for 3D (three dimensional) cell culture and related applications.
The MAPTrixTM hydrogel products are composed of two components: MAPTrix™ ECM, a mussel adhesive protein based extracellular matrix (ECM) mimetic; and, MAPTrix™ Link, a multi-arm polyethylene glycol derivative.
A key difference between MAPTrix™ based hydrogel products and currently available products is that the MAPTrix™ hydrogel products allow for the in situ formation of a “well-defined three dimensional environment”. MAPTrix hydrogel products generate well-defined 3D environments in terms of both biochemical composition; and, physical properties such as pore size and matrix elasticity of the hydrogel under physiological pH conditions. For example, a scientist can readily create a biochemically well-defined three dimensional ECM with use of either a single MAPTrix™ ECM product or a combination of MAPTrix™ ECM products. Kollodis currently provides several dozen different MAPTrix™ products in its MAPTrix™ ECM line of products.
Due to the extracellular matrix (ECM) derived peptide motifs recombinantly incorporated into Kollodis' mussel adhesive proteins (for example, the RGD structural motif in fibronectin), use of the MAPTrix™ ECM products can reduce or eliminate the serum requirement necessary for cell culture- as evidenced in primary hepatocyte or neural cell cultures conducted under serum free conditions. Depending upon the end-user's required extracellular protein (or more specifically, the peptide motif responsible for bioactivity), an appropriate MAPTrix™ ECM product can be selected to in situ create a three dimensional extracellular matrix environment suitable for their cell culture application.
Additionally, scientists can readily fine-tune or engineer the elasticity and/or pore size of the hydrogel by adjusting the concentration of MAPTrix™ ECM or MAPTrix™ Linker- particularly when they want to regulate a cell’s phenotype with such physical cues as the 3D matrix’s elasticity or pore size.
“We are absolutely delighted to commercialize our MAPTrix™ based hydrogel technology as a breakthrough solution addressing the unmet needs of a chemically defined environment for cell culture”, said Louis M. Scarmoutzos, Ph.D., Chief Executive Officer of Kollodis BioSciences. “The efficacy of our MAPTrix™ technology for 3D cell culture was demonstrated in endothelial tube formation. Simply mixing MAPTrix™ ECM with MAPTrix™ Link creates a 3D hydrogel matrix for tube formation within two hours”, he added.
Kollodis BioSciences, Inc.
Kollodis BioSciences, Inc. has successfully developed a proprietary recombinant protein expression system for the large scale production of various mussel adhesive proteins (MAP). Kollodis is dedicated to the development, manufacture, and sale of MAP-based smart biomaterials for biotech, medical and industrial applications. The Company's proprietary and patented MAPTrix™ platform technology recombinantly incorporates bioactive peptides into a mussel adhesive protein (MAP) for cell culture and related applications. Additional information is available on the Company's website at www.kollodis.com.
Kollodis BioSciences Announces Launch of MAP-based (Mussel Adhesive Protein) MAPTrix HyGelTM Line of Products: Chemically Defined Hydrogel Products for 3D Cell Culture Applications
edited by Kollodis BioSciences on 6/7/2010
|
|
6/6/2010 4:51:42 PM
|
topic:
VIDEO: in vivo versus 2D
QGel
Posts 8
|
A short video that compares 2D cell culture compared to an in vivo system.
Why study cells in 2D when life is in 3D?
CLICK HERE FOR THE VIDEO
|
|
6/6/2010 4:46:15 PM
|
topic:
How to Bioengineer QGel™ MT 3D Matrix
QGel
Posts 8
|
Check out this video on how QGel™ is bioengineered Video: QGel Bioengineered
For more info visit the QGel website
|
|
6/6/2010 4:44:16 PM
|
topic:
How to encapsulate cells with QGel™ MT 3D Matrix
QGel
Posts 8
|
A video on how to encapsulate cells with QGel can be viewed here: Encapsulating Cells in 3D with QGel
|
|
6/6/2010 4:39:26 PM
|
topic:
Imaging with alginate
QGel
Posts 8
|
Sometimes alginate is rather difficult to image because of its physical properties, but perhaps 1mm is a bit too thick. Also check the cell density - if it is too dense it could play a role.
You may wish to consider using QGel, a new hydrogel that results in excellent confocal images. Have a look at a confocal image of fibroblasts in QGel HERE
edited by QGel on 6/7/2010
|
|
pages:
1 2
|