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6/21/2010 10:22:44 AM
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topic:
JCB Interview with Joan Brugge
science.editor
Posts 45
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Journal of Cell Biology, 14 June, 189 (6): 922
Key Content: "We've been working on understanding the processes that drive these different phenotypic histologies, using a breast cancer model system we adapted from Mina Bissell's 3D cell culture work. Most recently, we've been studying the mechanisms that are responsible for the ability of tumor cells to survive without attachment to the extracellular matrix. Our 3D model system had shown us that acini start out as a solid sphere of cells. The hollow lumen is generated during morphogenesis through selective apoptosis of the inner cells. But we noticed that if you blocked apoptosis in these cells, they still died."
Original Source: http://jcb.rupress.org/
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6/18/2010 1:40:15 PM
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topic:
CPW: A strategy for fabrication of a 3D Tissue...
3dcc blogger
Posts 13
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This week I chose "A strategy for fabrication of a three-dimensional tissue construct containing uniformly distributed embryoid body-derived cells as a cardiac patch" for Cool Paper of the Week!
This group used a series of really neat techniques to create mouse embryonic cell sheets. These researchers first cultured the cells on a hydrogel, then removed the cell sheet layers and "sandwiched" the cell sheets between layers of bovine acellular tissue to create a 3D tissue construct. Check out this paper for more details!
http://www.3dcellculture.com/Search_Results/id/9134
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6/11/2010 1:00:33 PM
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topic:
Bristol U: 2 Postdocs CLOSED
science.editor
Posts 45
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Key Content: Two three-year postdoctoral positions are available from October 2010 in Prof Dek Woolfson’s laboratory. The project is to design, produce and develop peptides that self-assemble into alpha-helical hydrogels, and apply these in 3D cell culture. The work builds on the group’s success in designing peptide-based biomaterials (Nature Materials 8:596 (2009), and aims to move this onto real-life applications in tissue engineering and towards the clinic ....
Original Source: http://www.youngbrigades.com/scholarships-and-higher-education/uk2-postdoctoral-position-in-synthetic-biology-and-tissue-engineering.html
Nature Jobs: http://www.nature.com/naturejobs/science/jobs/147652-Postdoctoral-Research-Assistants-in-Synthetic-Biology-and-Tissue-Engineering-two-posts-reference-15501
edited by science.editor on 6/21/2010
edited by science.editor on 7/14/2010
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6/9/2010 4:09:36 PM
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topic:
Test QGel's new 3D synthetic hydrogel
QGel
Posts 12
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Start a project and collaborate with QGel and get free samples of our matrix to grow cells in 3D. QGel has opened a call for projects.
The process is simple:
1- You submit a brief description of your project online
2- QGel experts meet weekly to evaluate project submissions
3- You are notified of project acceptance and your sample material is shipped
note. There are a limited number of vials QGel has allocated for these projects. Be sure to submit your project soon.
To find out more information on how to submit your 3D Cell Culture project using QGel, visit the website Collaborate with QGel.
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6/9/2010 7:35:40 AM
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topic:
TEXTBOOK: 3D Cell-Based Biosensors in Drug Discov
science.editor
Posts 45
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3D Cell-Based Biosensors in Drug Discovery Programs: Microtissue Engineering for High Throughput Screening
William S. Kisaalita, University of Georgia, Athens, USA
Price: $129.95
Cat. #: 73494
ISBN: 9781420073492
ISBN 10: 1420073494
Publication Date: June 23, 2010
Number of Pages: 404
Key content:
* Provides a deep comparative analysis of 2D and 3D cultures
* Uses data from peer-reviewed publications to conclusively provide the justification for use of 3D cell cultures in cell-based biosensors (assays) for high throughput screening
* Includes difficult-to-find information such as patents, cell lines or types commonly used in drug discovery programs, and their origins
* Addresses research needs mainly in areas of detection and scaffold engineering
* Describes microenvironment factors for creating phenotypes that resemble their in vivo counterparts
* Incorporates case studies in support of 3D cell-based biosensor adoption in drug discovery programs
Original Source: http://www.crcpress.com/product/isbn/9781420073492
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6/8/2010 9:46:11 AM
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topic:
The Scientist.com: 3D Science
science.editor
Posts 45
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Volume 24 | Issue 6 | Page 27
Date: 2010-06-01
KEY CONTENT: The role of dimensionality is a growing field in cell biology, but many labs cannot afford new tools that let cells grow in three dimensions—which could, in theory, better represent what happens in vivo. As a member of F1000’s Faculty of Cell Biology, Ken Yamada at the National Institute of Dental and Craniofacial Research flagged a recent PNAS study that described an easy alternative for scientists who want to explore 3D cell culture: paper (106:18457–62, 2009).
TS: Cells are normally grown in a single layer on a plastic surface or in suspension. What’s wrong with this approach?
KY: It’s obviously spatially artificial. You lose normal three-dimensional architecture and the normal interactions of cells with their surroundings, both with the matrix and other cells. The result is usually altered cell shape and gene expression. It seems that dimensionality by itself affects cell behavior.
The other thing about cells grown on a plastic surface is that there’s a major difference in stiffness. The substrate cannot be deformed, whereas in vivo you have elasticity of the matrix.
See article for remainder ....
Original Source: http://www.the-scientist.com/article/display/57462/
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6/7/2010 8:59:41 AM
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topic:
Kollodis BioSciences Launches 3D Hydrogel Products
Kollodis BioSciences
Posts 1
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Kollodis BioSciences Announces Launch of MAP-based (Mussel Adhesive Protein) MAPTrix HyGelTM Line of Products: Chemically Defined Hydrogel Products for 3D Cell Culture Applications
(MALDEN, MA) June 3, 2010 - Kollodis BioSciences, Inc. has announced the launch of its novel MAPTrix HyGelTM line of products. MAPTrix HyGelTM is a chemically well-defined product based on Kollodis BioSciences' proprietary recombinant Mussel Adhesive Protein technology. MAPTrix HyGelTM is an in situ formable hydrogel product designed for 3D (three dimensional) cell culture and related applications.
The MAPTrixTM hydrogel products are composed of two components: MAPTrix™ ECM, a mussel adhesive protein based extracellular matrix (ECM) mimetic; and, MAPTrix™ Link, a multi-arm polyethylene glycol derivative.
A key difference between MAPTrix™ based hydrogel products and currently available products is that the MAPTrix™ hydrogel products allow for the in situ formation of a “well-defined three dimensional environment”. MAPTrix hydrogel products generate well-defined 3D environments in terms of both biochemical composition; and, physical properties such as pore size and matrix elasticity of the hydrogel under physiological pH conditions. For example, a scientist can readily create a biochemically well-defined three dimensional ECM with use of either a single MAPTrix™ ECM product or a combination of MAPTrix™ ECM products. Kollodis currently provides several dozen different MAPTrix™ products in its MAPTrix™ ECM line of products.
Due to the extracellular matrix (ECM) derived peptide motifs recombinantly incorporated into Kollodis' mussel adhesive proteins (for example, the RGD structural motif in fibronectin), use of the MAPTrix™ ECM products can reduce or eliminate the serum requirement necessary for cell culture- as evidenced in primary hepatocyte or neural cell cultures conducted under serum free conditions. Depending upon the end-user's required extracellular protein (or more specifically, the peptide motif responsible for bioactivity), an appropriate MAPTrix™ ECM product can be selected to in situ create a three dimensional extracellular matrix environment suitable for their cell culture application.
Additionally, scientists can readily fine-tune or engineer the elasticity and/or pore size of the hydrogel by adjusting the concentration of MAPTrix™ ECM or MAPTrix™ Linker- particularly when they want to regulate a cell’s phenotype with such physical cues as the 3D matrix’s elasticity or pore size.
“We are absolutely delighted to commercialize our MAPTrix™ based hydrogel technology as a breakthrough solution addressing the unmet needs of a chemically defined environment for cell culture”, said Louis M. Scarmoutzos, Ph.D., Chief Executive Officer of Kollodis BioSciences. “The efficacy of our MAPTrix™ technology for 3D cell culture was demonstrated in endothelial tube formation. Simply mixing MAPTrix™ ECM with MAPTrix™ Link creates a 3D hydrogel matrix for tube formation within two hours”, he added.
Kollodis BioSciences, Inc.
Kollodis BioSciences, Inc. has successfully developed a proprietary recombinant protein expression system for the large scale production of various mussel adhesive proteins (MAP). Kollodis is dedicated to the development, manufacture, and sale of MAP-based smart biomaterials for biotech, medical and industrial applications. The Company's proprietary and patented MAPTrix™ platform technology recombinantly incorporates bioactive peptides into a mussel adhesive protein (MAP) for cell culture and related applications. Additional information is available on the Company's website at www.kollodis.com.
Kollodis BioSciences Announces Launch of MAP-based (Mussel Adhesive Protein) MAPTrix HyGelTM Line of Products: Chemically Defined Hydrogel Products for 3D Cell Culture Applications
edited by Kollodis BioSciences on 6/7/2010
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6/6/2010 4:51:42 PM
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topic:
VIDEO: in vivo versus 2D
QGel
Posts 12
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A short video that compares 2D cell culture compared to an in vivo system.
Why study cells in 2D when life is in 3D?
CLICK HERE FOR THE VIDEO
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6/6/2010 4:46:15 PM
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topic:
How to Bioengineer QGel™ MT 3D Matrix
QGel
Posts 12
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Check out this video on how QGel™ is bioengineered Video: QGel Bioengineered
For more info visit the QGel website
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6/6/2010 4:44:16 PM
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topic:
How to encapsulate cells with QGel™ MT 3D Matrix
QGel
Posts 12
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A video on how to encapsulate cells with QGel can be viewed here: Encapsulating Cells in 3D with QGel
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