http://www.3dcellculture.com/RSSReal Life Happens in 3D. So should your Cell Culture.en-usTue, 07 Feb 2012 04:12:33 ESTTue, 07 Feb 2012 04:12:33 ESTWebSalesToolmatt@3dcellculture.com (3DCellCulture.com)support@websalestool.com (WST Admin)Thu, 02 Feb 2012 14:01:53 ESThttp://www.3dcellculture.com/search_results/id/9996The microenvironment plays a significant role in human cancer progression. However, the role of the tumor microenvironment in the epigenetic control of genes critical to cancer progression remains unclear. As transient E-cadherin expression is central to many stages of neoplasia and is sensitive to regulation by the microenvironment, we have studied if microenvironmental control of E-cadherin expression is linked to transient epigenetic regulation of its promoter, contributing to the unstable an...http://www.3dcellculture.com/search_results/id/9996Thu, 19 Jan 2012 11:40:23 ESThttp://www.3dcellculture.com/search_results/id/9995A three-dimensional culture system supports the development of primate preantral follicles to the antral stage with appreciable steroid production. This study assessed i) whether in vitro developmental competence of follicles is age dependent, ii) the role of gonadotropins and insulin in supporting folliculogenesis, and iii) anti-Mullerian hormone (AMH) and vascular endothelial growth factor (VEGF) production by growing follicles. Ovaries were obtained from prepubertal, young, and older adult rh...http://www.3dcellculture.com/search_results/id/9995Thu, 12 Jan 2012 09:43:34 ESThttp://www.3dcellculture.com/search_results/id/9994For the purpose of fetal membrane healing, amnion mesenchymal cells were embedded in three-dimensional (3D) collagen I, and amnion epithelial cells were placed on top of it to build the amniotic cells/collagen I matrix complex to simulate the architecture of native amnion. Morphology of cell cultured on 3D collagen I was visualized by F-actin filaments incubated with rhodamine-labelled phalloidin. Cell ultrastructure of amnion epithelial cells and amnion mesenchymal cells was visualized by scann...http://www.3dcellculture.com/search_results/id/9994Thu, 12 Jan 2012 09:43:32 ESThttp://www.3dcellculture.com/search_results/id/9993Recently, in situ tissue engineering has emerged as a new approach to obtain autologous, living replacement tissues with off-the-shelf availability. The method is based on the use of an instructive biodegradable scaffold that is capable of repopulation with host cells in situ and subsequent tissue formation. This approach imposes high demands on scaffold properties. For cardiovascular grafts, the repopulation with endogenous cells from the circulation is further hypothesized to be influenced by ...http://www.3dcellculture.com/search_results/id/9993Thu, 12 Jan 2012 09:43:32 ESThttp://www.3dcellculture.com/search_results/id/9992This study attempted to use collagen-Matrigel as extracellular matrix (ECM) to supply cells with three-dimensional (3D) culture condition and employ alginate-poly-l-lysine-alginate (APA) microcapsules to control the formation of alveolus-like structure in vitro. We tested mice foetal pulmonary cells (FPCs) by immunohistochemistry after 2D culture. The alveolus-like structure was reconstructed by seeding FPCs in collagen-Matrigel mixed with APA microcapsules 1.5 ml. A self-made mould was used to ...http://www.3dcellculture.com/search_results/id/9992Thu, 12 Jan 2012 09:43:30 ESThttp://www.3dcellculture.com/search_results/id/9991Inverse opal scaffolds have recently emerged as a novel class of scaffolds with uniform and controllable pore sizes for tissue engineering to provide better nutrient transport, a uniform cell distribution, and an adjustable microenvironment for cell differentiation. However, when the pore size of the scaffold is much larger than the dimension of a cell, the cell actually encounters a local 2D environment and the void space associated with the pore can not be efficiently utilized. Here, we demons...http://www.3dcellculture.com/search_results/id/9991Fri, 06 Jan 2012 11:54:56 ESThttp://www.3dcellculture.com/search_results/id/9990Fabrication of 3-D highly porous, bioactive, and mechanically competent scaffolds represents a significant challenge of bone tissue engineering. In this work, Bioglass(R)-derived glass-ceramic scaffolds actually fulfilling this complex set of requirements were successfully produced through the sponge replication method. Scaffold processing parameters and sintering treatment were carefully designed in order to obtain final porous bodies with pore content (porosity above 70 %vol), trabecular archi...http://www.3dcellculture.com/search_results/id/9990Fri, 06 Jan 2012 11:54:55 ESThttp://www.3dcellculture.com/search_results/id/9989BackgroundRecent studies identified mesenchymal stem cells (MSC) as major players in vascular remodelling and the sub-endothelial compartment as the stem cell niche. The uraemic microenvironment predisposes to increased levels of reactive oxygen species, accelerated ageing of endothelial cells (EC) and vascular sclerosis.MethodsWe generated an in vitro model of a vascular niche consisting of a three-dimensional collagen I/III gel containing MSC and EC. We recapitulated a uraemic microenvironment...http://www.3dcellculture.com/search_results/id/9989Fri, 06 Jan 2012 11:54:54 ESThttp://www.3dcellculture.com/search_results/id/9988We report the adaptation of dendrimer-based nonviral expression system for ciliary neurotrophic factor (CNTF) overproduction in human mesenchymal stem cells (hMSCs) embedded into fibrin-based three-dimensional (3D) matrix. Time-restricted neurotrophin expression enables autologous adult stem cells for additional trophic support and increases their therapeutic potential in neuroregeneration applications. Polyamidoamine (PAMAM)-NH(2) dendrimers of fourth generation effectively provided virus-free ...http://www.3dcellculture.com/search_results/id/9988Thu, 29 Dec 2011 12:48:13 ESThttp://www.3dcellculture.com/search_results/id/9987OBJECTIVE: To examine the impact of hormones used for controlled ovarian hyperstimulation (COH) on normal and malignant breast cell growth and proliferation. DESIGN: In vitro study of cultured normal and malignant breast cell lines. SETTING: Academic medical center. PATIENT(S): None. INTERVENTION(S): Normal and malignant breast cell lines cultured in two- and three-dimensional (2D and 3D) systems and treated with follicle-stimulating hormone (FSH), luteinizing hormone (LH), or FSH with LH or hum...http://www.3dcellculture.com/search_results/id/9987Thu, 29 Dec 2011 12:48:12 ESThttp://www.3dcellculture.com/search_results/id/9986The focus on creating tissue engineered constructs of clinically relevant sizes requires new approaches for monitoring construct health during tissue development. A few key requirements are that the technology be in situ, non-invasive, and provide temporal and spatial information. In this work, we demonstrate that optical coherence microscopy (OCM) can be used to assess cell viability without the addition of exogenous probes in three-dimensional (3D) tissue scaffolds maintained under standard cu...http://www.3dcellculture.com/search_results/id/9986Thu, 29 Dec 2011 12:48:12 ESThttp://www.3dcellculture.com/search_results/id/9985Cells in a functional tissue display a highly interactive relation with their neighboring cells and associated biochemical milieu. Serious disruption in the existing homeostatic balance in the extra-cellular matrix (ECM) may lead to abnormal response by the cells. With insufficient understanding of Cell-ECM interaction and in absence of simple tools for in vitro cell-culture in 3D, we still have to rely on the data generated by growing cells in 2D. In order to comprehend Cell-ECM dynamics it is ...http://www.3dcellculture.com/search_results/id/9985Thu, 29 Dec 2011 12:48:11 ESThttp://www.3dcellculture.com/search_results/id/9984A significant stumbling block in the creation of functional three-dimensional (3D) engineered tissues is the proper vascularization of the constructs. Furthermore, in the context of electrospinning, the development of 3D constructs using this technique has been hindered by the limited infiltration of cells into their structure. In an attempt to address these issues, a hybrid mesh of poly (varepsilon-caprolactone)-collagen blend (PCL/Col) and hyaluronic acid (HA) hydrogel, Heprasil was created vi...http://www.3dcellculture.com/search_results/id/9984Thu, 29 Dec 2011 12:48:10 ESThttp://www.3dcellculture.com/search_results/id/9983Realizing the potential of human pluripotent stem cell (hPSC)-based therapy requires the development of defined scalable culture systems with efficient expansion, differentiation and isolation protocols. We report an engineered 3D microfiber system that efficiently supports long-term hPSCs self-renewal under chemically defined conditions. The unique feature of this system lies in the application of a 3D ECM-like environment in which cells are embedded, that affords: (i) uniform high cell loading...http://www.3dcellculture.com/search_results/id/9983Thu, 29 Dec 2011 12:48:10 ESThttp://www.3dcellculture.com/search_results/id/9982Three-dimensional (3D) engineered tissue constructs are a novel and promising approach to tissue repair and regeneration. 3D tissue constructs have the ability to restore form and function to damaged soft tissue unlike previous methods, such as plastic surgery, which are able to restore only form, leaving the function of the soft tissue often compromised. In this study, we seeded murine myoblasts (C2C12) into a collagen composite scaffold and cultured the scaffold in a roller bottle cell culture...http://www.3dcellculture.com/search_results/id/9982Thu, 29 Dec 2011 12:48:09 ESThttp://www.3dcellculture.com/search_results/id/9981Current therapeutic angiogenesis strategies are focused on the development of biologically responsive scaffolds that can deliver multiple angiogenic cytokines and/or cells in ischemic regions. Herein we report on a novel electrospinning approach to fabricate cytokine-loaded nanofibrous scaffolds with a patterned architecture to promote angiogenesis. Electrospun scaffolds with a controlled fiber architecture were prepared from gelatin solutions by controlling the feed rate, needle to collector (N...http://www.3dcellculture.com/search_results/id/9981Wed, 28 Dec 2011 10:31:16 ESThttp://www.3dcellculture.com/search_results/id/9980While stem cell niches in vivo are complex three-dimensional (3D) microenvironments, the relationship between the dimensionality of the niche to its function is unknown. We have created a 3D microenvironment through electrospinning to study the impact of geometry and different extracellular proteins on the development of cardiac progenitor cells (Flk-1(+)) from resident stem cells and their differentiation into functional cardiovascular cells. We have investigated the effect of collagen IV, fibr...http://www.3dcellculture.com/search_results/id/9980Wed, 28 Dec 2011 10:31:15 ESThttp://www.3dcellculture.com/search_results/id/9979PURPOSE: Drug resistance in melanoma is commonly attributed to ineffective apoptosis pathways. Inhibiting antiapoptotic BCL-2 and its relatives is an attractive strategy for sensitizing lymphoid malignancies to drugs but it has been largely unsuccessful for melanoma and other solid tumors. ABT-737, a small-molecule BH3-mimetic, selectively inhibits BCL-2, BCL-XL and BCL-w and shows promise for treating leukemia, lymphoma and small cell lung cancer. Melanoma cells are insensitive to ABT-737 but M...http://www.3dcellculture.com/search_results/id/9979Wed, 28 Dec 2011 10:31:15 ESThttp://www.3dcellculture.com/search_results/id/9978In tissue engineering, chemical and topographical cues are normally developed using static cell cultures but then applied directly to tissue cultures in three dimensions (3D) and under perfusion. As human cells are very sensitive to changes in the culture environment, it is essential to evaluate the performance of any such cues in a perfused environment before they are applied to tissue engineering. Thus, the aim of this research was to bridge the gap between static and perfusion cultures by add...http://www.3dcellculture.com/search_results/id/9978Wed, 28 Dec 2011 10:30:42 ESThttp://www.3dcellculture.com/search_results/id/9977A three-dimensional (3D) cell culture module was used to test the requirements of scaffold material properties for improved bone cell ingrowth. For this purpose polycarbonate (PC) samples were treated with microwave-excited ammonia plasma in a defined time frame of 1–300?s and the influence on initial attachment of osteoblast-like cells was determined. Perforated PC slices were treated by 30?s of ammonia plasma and mounted on a clamp for 3D cell ingrowth. Plasma treatment conditions were ascerta...http://www.3dcellculture.com/search_results/id/9977Wed, 28 Dec 2011 10:30:08 ESThttp://www.3dcellculture.com/search_results/id/9976Tracheal tumours can be surgically resected but most are an inoperable size at the time of diagnosis; therefore, new therapeutic options are needed. We report the clinical transplantation of the tracheobronchial airway with a stem-cell-seeded bioartificial nanocomposite. A 36-year-old male patient, previously treated with debulking surgery and radiation therapy, presented with recurrent primary cancer of the distal trachea and main bronchi. After complete tumour resection, the airway was replace...http://www.3dcellculture.com/search_results/id/9976Fri, 16 Dec 2011 14:23:23 ESThttp://www.3dcellculture.com/search_results/id/9975BACKGROUND: In vitro cell observation has been widely used by biologists and pharmacologists for screening molecule-induced effects on cancer cells. Computer-assisted time-lapse microscopy enables automated live cell imaging in vitro, enabling cell behavior characterization through image analysis, in particular regarding cell migration. In this context, 3D cell assays in transparent matrix gels have been developed to provide more realistic in vitro 3D environments for monitoring cell migration (...http://www.3dcellculture.com/search_results/id/9975Fri, 16 Dec 2011 14:23:22 ESThttp://www.3dcellculture.com/search_results/id/9974We tested the hypothesis that the osteoblast differentiation status of bone marrow stem cells (BMSC) combined with a three-dimensional (3D) structure modulates bone formation when autogenously implanted. Rat BMSC were aspirated, expanded and seeded into a 3D composite of poly(lactide-co-glycolide) and calcium phosphate (PLGA/CaP) to produce a hybrid biomaterial. Calvarial defects were implanted with: (1) scaffold without cells (SC/NC); (2) scaffold and BMSC (SC+BMSC); (3) scaffold and osteoblast...http://www.3dcellculture.com/search_results/id/9974Fri, 16 Dec 2011 14:23:20 ESThttp://www.3dcellculture.com/search_results/id/9973Till date, no bioartificial liver (BAL) procedure has obtained FDA approval or widespread clinical acceptance, mainly because of multifactorial limitations such as the use of microscale or undefined biomaterials, indirect and lower oxygenation levels in liver cells, short-term undesirable functions, and a lack of 3D interaction of growth factor/cytokine signaling in liver cells. To overcome preclinical limitations, primary rat liver cells were cultured on a naturally self-assembling peptide nano...http://www.3dcellculture.com/search_results/id/9973Fri, 16 Dec 2011 14:23:18 ESThttp://www.3dcellculture.com/search_results/id/9972We previously reported the development of a simple, user-friendly, and versatile 384 hanging drop array plate for 3D spheroid culture and the importance of utilizing 3D cellular models in anti-cancer drug sensitivity testing. The 384 hanging drop array plate allows for high-throughput capabilities and offers significant improvements over existing 3D spheroid culture methods. To allow for practical 3D cell-based high-throughput screening and enable broader use of the plate, we characterize the ro...http://www.3dcellculture.com/search_results/id/9972