http://www.3dcellculture.com/RSSReal Life Happens in 3D. So should your Cell Culture.en-usFri, 30 Jul 2010 07:31:12 ESTFri, 29 Jul 2010 15:05:41 ESTWebSalesToolmatt@3dcellculture.com (3DCellCulture.com)support@websalestool.com (WST Admin)Thu, 29 Jul 2010 15:05:41 ESThttp://www.3dcellculture.com/search_results/id/9220Abstract Cell-monolayer-based assays for chemotherapeutic drug discovery have proven to be highly artificial compared with physiological systems. The objective of this study was to culture cancer cells in a simple 3-dimensional (3D) collagen gel model to study the antiproliferative activity of known lung cancer drugs. The validity of our 3D model was tested by measuring the activity of 10 lung cancer drugs (Paclitaxel, Alimta, Zactima, Doxorubicin, Vinorelbine, Gemcitabine, 17AAg, Cisplatin, and...http://www.3dcellculture.com/search_results/id/9220Thu, 29 Jul 2010 15:05:41 ESThttp://www.3dcellculture.com/search_results/id/9219Stalled cell division in precursor bone cells and reduced osteoblast function are considered responsible for the microgravity-induced bone loss observed during spaceflight. However, underlying molecular mechanisms remains unraveled. Having overcome technological difficulties associated with flying cells in a space mission, we present the first report on the behavior of the potentially osteogenic murine bone marrow stromal cells (BMSC) in a 3D culture system, flown inside the KUBIK aboard space m...http://www.3dcellculture.com/search_results/id/9219Thu, 29 Jul 2010 15:05:40 ESThttp://www.3dcellculture.com/search_results/id/9218To establish an ex vivo expansion method of haematopoietic progenitor cells (HPCs) and erythroid cells, three-dimensional (3D) cultures of mouse bone marrow cells were performed, employing a porous polyvinyl formal (PVF) resin as a scaffold. In these cultures, the effects of oxygen concentration and co-cultures with stromal cells on the expansion of HPCs and erythroid cells were investigated. When bone marrow cells were cultured under 3D conditions, HPCs and erythroid cells expanded without supp...http://www.3dcellculture.com/search_results/id/9218Thu, 29 Jul 2010 15:05:39 ESThttp://www.3dcellculture.com/search_results/id/9217Scaffold-free three-dimensional (3D) cultures provide clinical potential in cartilage regeneration. The purpose of this study was to characterize a scaffold-free 3D membrane-based culture system, in which human articular chondrocytes were cultivated on a cellulose acetate membrane filter, and compare it to pellet and monolayer cultures. Chondrocytes were expanded in monolayer culture for up to 5 passages, transferred to membrane-based or pellet cultures and harvested after 7 or 21 days. The chon...http://www.3dcellculture.com/search_results/id/9217Thu, 29 Jul 2010 15:05:38 ESThttp://www.3dcellculture.com/search_results/id/9216Drug discovery programmes require accurate in vitro systems for drug screening and testing. Traditional cell culture makes use of 2D (two-dimensional) surfaces for ex vivo cell growth. In such environments, cells are forced to adopt unnatural characteristics, including aberrant flattened morphologies. Therefore there is a strong demand for new cell culture platforms which allow cells to grow and respond to their environment in a more realistic manner. The development of 3D (three-dimensional) al...http://www.3dcellculture.com/search_results/id/9216Thu, 29 Jul 2010 15:05:37 ESThttp://www.3dcellculture.com/search_results/id/9215Purpose: The purpose of this study was to determine whether osseous tissues engineered in three-dimensional (3D) environments preserved their mineralizing capacity and retained biologic characteristics when cultured on dental implant surfaces. Materials and Methods: Human preosteoblast cells were cultured in both 3D rotary wall vessels and on 2D tissue culture plastic plates for 3 days. Aggregates from the 3D chambers and cells from the 2D plates were collected and transferred to commercially pu...http://www.3dcellculture.com/search_results/id/9215Thu, 29 Jul 2010 15:05:37 ESThttp://www.3dcellculture.com/search_results/id/9214Intervertebral disc degeneration is considered a major source of low back pain. We therefore examined an absorbable polyglycolic acid (PGA) biomaterial for its utility to support disc tissue regeneration. Microdiscectomy for lumbar disc herniation was performed in six patients. Intervertebral disc cells were isolated and in vitro cell expansion was accomplished using human serum and FGF2. In a fibrin-hyaluronan solution, disc cells were loaded on PGA scaffolds and cultured for 2 weeks. Formation...http://www.3dcellculture.com/search_results/id/9214Thu, 29 Jul 2010 15:05:36 ESThttp://www.3dcellculture.com/search_results/id/9213BACKGROUND CONTEXT: Understanding gene expression patterns of disc cells in culture is important as we develop biologic therapies for disc degeneration. The objective of the present study was to determine if cells from more degenerated discs expressed different genes, or differed in their expression patterns, compared with patterns of cells from healthier discs. PURPOSE: To determine if annulus cells from more degenerated discs expressed different gene expression patterns compared with patterns ...http://www.3dcellculture.com/search_results/id/9213Thu, 29 Jul 2010 15:05:35 ESThttp://www.3dcellculture.com/search_results/id/9212In the present in vitro study, the axon growth promoting effects of human neural progenitor-derived astrocytes (hNP-AC) were investigated in simple 2D- as well as in more complex 3D-culture systems. The interactions of the hNP-AC with migrating Schwann cells and fibroblasts were also studied. hNP-AC were found to promote extensive dorsal root ganglion axon regeneration in 2D cultures, being even greater than that observed on the positive control, laminin-coated substrate. Contact-mediated mechan...http://www.3dcellculture.com/search_results/id/9212Thu, 29 Jul 2010 15:05:35 ESThttp://www.3dcellculture.com/search_results/id/9211BACKGROUND: For current tissue engineering or regenerative medicine strategies, chondrocyte- or mesenchymal stem cell (MSC)-seeded constructs are typically cultured in normoxic conditions (20% oxygen). However, within the knee joint capsule a lower oxygen tension exists. OBJECTIVE: The objective of this study was to investigate how chondrocytes and infrapatellar fad pad derived MSCs will respond to a low oxygen (5%) environment in 3D agarose culture. Our hypothesis was that culture in a low oxyg...http://www.3dcellculture.com/search_results/id/9211Thu, 29 Jul 2010 15:05:01 ESThttp://www.3dcellculture.com/search_results/id/9210A cell`s microenvironment plays a primary role in defining cell fate during tissue development, physiological function, and pathological dysfunction. Understanding the key components and interactions within these microenvironments is critical for effective use of stem cells for disease modeling and therapeutic applications. Yet cell microenvironments are difficult to study, as there are tens or hundreds of parameters that can influence cell behavior simultaneously. Additionally, parameters such ...http://www.3dcellculture.com/search_results/id/9210Thu, 29 Jul 2010 15:04:26 ESThttp://www.3dcellculture.com/search_results/id/9209Three-dimensional (3D) cellular assays closely mimic the in vivo milieu, providing a rapid, inexpensive system for screening drug candidates for toxicity or efficacy in the early stages of drug discovery. However, 3D culture systems may suffer from mass transfer limitations, particularly in delivery of large polypeptide or nucleic acid compounds. Nucleic acids (e.g., genes, silencing RNA) are of particular interest both as potential therapeutics and due to a desire to modulate the gene-expressio...http://www.3dcellculture.com/search_results/id/9209Thu, 29 Jul 2010 15:03:56 ESThttp://www.3dcellculture.com/search_results/id/9208The promising potential of a RAD-16 self-assembly-peptide hydrogel as a scaffold for tissue-engineered cartilage was investigated. Within 3 weeks of in vitro culture, chondrocytes within the hydrogel produced a high amount of GAG and type-II collagen, which are the components of cartilage-specific extracellular matrix (ECM). With the culture time increased, toluidine-blue staining for GAG and immuno-histochemistry staining for type-II collagen of the chondrocytes-hydrogel composites became more ...http://www.3dcellculture.com/search_results/id/9208Thu, 29 Jul 2010 15:03:21 ESThttp://www.3dcellculture.com/search_results/id/9207The development of novel breast cancer therapies depends on appropriate experimental models of disease pathogenesis. In recent years, there has been a proliferation of sophisticated models for breast cancer research. We describe here novel models that have particular relevance for drug development, and discuss the advantages and disadvantages of each model with respect to the various aspects of breast cancer pathogenesis....http://www.3dcellculture.com/search_results/id/9207Thu, 29 Jul 2010 15:02:12 ESThttp://www.3dcellculture.com/search_results/id/9206Ocular angiogenesis is the leading cause of blindness and is associated with diabetic retinopathy and age-related macular degeneration. We describe, in this report, our preliminary studies using a horizontally rotating bioreactor (HRB), developed by the National Aeronautics and Space Administration (NASA), to explore growth and differentiation-associated events in the early phase of ocular angiogenesis. Human retinal (HRet) cells and bovine endothelial cells (ECs) were cocultured on laminin-coat...http://www.3dcellculture.com/search_results/id/9206Thu, 29 Jul 2010 15:01:41 ESThttp://www.3dcellculture.com/search_results/id/9205Embryonic stem (ES)-cell-derived lineage-specific stem cells, for example, hematopoietic stem cells, could provide a potentially unlimited source for transplantable cells, especially for cell-based therapies. However, reproducible methods must be developed to maximize and scale-up ES cell differentiation to produce clinically relevant numbers of therapeutic cells. Bioreactor-based dynamic culture conditions are amenable to large-scale cell production, but few studies have evaluated how various b...http://www.3dcellculture.com/search_results/id/9205Thu, 29 Jul 2010 15:00:36 ESThttp://www.3dcellculture.com/search_results/id/9204We report here the first attempt to produce and use whole acellular (AC) lung as a matrix to support development of engineered lung tissue from murine embryonic stem cells (mESCs). We compared the influence of AC lung, Gelfoam, Matrigel, and a collagen I hydrogel matrix on the mESC attachment, differentiation, and subsequent formation of complex tissue. We found that AC lung allowed for better retention of cells with more differentiation of mESCs into epithelial and endothelial lineages. In cons...http://www.3dcellculture.com/search_results/id/9204Thu, 29 Jul 2010 14:59:53 ESThttp://www.3dcellculture.com/search_results/id/9203The promise of human embryonic stem cells (hESCs) to provide an unlimited supply of cells for cell therapy and tissue engineering depends on the availability of a controllable bioprocess for their expansion and differentiation. We describe for the first time the formation of differentiating human embryoid bodies (hEBs) in rotating bioreactors to try and control their agglomeration. The efficacy of the dynamic process compared to static cultivation in Petri dishes was analyzed with respect to the...http://www.3dcellculture.com/search_results/id/9203Thu, 29 Jul 2010 14:59:21 ESThttp://www.3dcellculture.com/search_results/id/9202When will embryonic stem cells reach the clinic? The answer is simple 2013 not soon! To produce large quantities of homogeneous tissue for transplantation, without feeder layers, and with the appropriate recipient`s immunological phenotype, is a significant scientific hindrance, although adult stem (ADS) cells provide an alternative, more ethically acceptable, source. The annual global 100 million human birth rate proposes umbilical cord blood (UCB) as the largest untouched stem cell source, wit...http://www.3dcellculture.com/search_results/id/9202Thu, 29 Jul 2010 14:57:58 ESThttp://www.3dcellculture.com/search_results/id/9201Stem cells offer the distinct prospect of changing the face of human medicine. However, although they have potential to form different tissues, are still in the early stages of development as therapeutic interventions. The three most used stem cell sources are umbilical cord blood, bone marrow and human embryos. Whilst, cord blood is now used to treat over 70 disorders, at the time of writing this manuscript, not a single disease has been overcome or ameliorated using human embryonic stem cells....http://www.3dcellculture.com/search_results/id/9201Thu, 29 Jul 2010 14:57:21 ESThttp://www.3dcellculture.com/search_results/id/9200Cell interactions with the extracellular matrix (ECM) play a critical role in their physiology. Here, we sought to determine the role of exogenous and endogenous ECM in the differentiation of nonhuman primate ESCs. We evaluated cell differentiation from expression of lineage gene mRNA and proteins using real-time polymerase chain reaction and immunohistochemistry. We found that ESCs that attached to and spread upon highly adhesive collagen do not differentiate efficiently, whereas on the less ad...http://www.3dcellculture.com/search_results/id/9200Thu, 29 Jul 2010 14:56:11 ESThttp://www.3dcellculture.com/search_results/id/9199Embryonic stem cell (ESC) culture is fragmented and laborious and involves operator decisions. Most protocols consist of 3 individual steps: maintenance, embryoid body (EB) formation, and differentiation. Integration will assist automation, ultimately aiding scale-up to clinically relevant numbers. These problems were addressed by encapsulating undifferentiated murine ESCs (mESCs) in 1.1% (w/v) low-viscosity alginic acid, 0.1% (v/v) porcine gelatin hydrogel beads (d = 2.3 mm). Six hundred beads ...http://www.3dcellculture.com/search_results/id/9199Thu, 29 Jul 2010 14:55:35 ESThttp://www.3dcellculture.com/search_results/id/9198Neural stem cells and neural progenitors (NSCs/NPs) are capable of self-renewal and can give rise to both neurons and glia. Such cells have been isolated from the embryonic brain and immobilized in three dimensional collagen gels. The collagen-entrapped NSCs/NPs recapitulate CNS stem cell development and form functional synapses and neuronal circuits. However, the cell-collagen constructs from static conditions contain hypoxic, necrotic cores and the cells are short-lived. In the present study, ...http://www.3dcellculture.com/search_results/id/9198Thu, 29 Jul 2010 14:53:36 ESThttp://www.3dcellculture.com/search_results/id/9197Dexamethasone (Dex) is used widely to induce differentiation in human mesenchymal stem cells (hMSCs); however, using a pharmaceutical agent to stimulate hMSC differentiation is not the best choice for engineered tissue transplantation due to potential side-effects. The goal of the present study was to investigate the effects of dynamic compressive loading on differentiation and mineralized matrix production of hMSCs in 3D polyurethane scaffolds, using a loading regimen previously shown to stimul...http://www.3dcellculture.com/search_results/id/9197Thu, 29 Jul 2010 14:53:35 ESThttp://www.3dcellculture.com/search_results/id/9196Serum albumin was conjugated to Poly-(ethylene glycol) (PEG) and cross-linked to form mono-PEGylated albumin hydrogels. These hydrogels were used as a basis for drug carrying tissue engineered scaffold materials, based on the natural affinity of various drugs and compounds to the tethered albumin in the polymer network. The results of the drug release validation experiments showed that the release kinetics of the drugs from the mono-PEGylated albumin hydrogels were controlled by the molecular we...http://www.3dcellculture.com/search_results/id/9196