Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration

Periodical: Biotechnol Lett ISBN: 0141-5492 (Print)  Number: 3  Date: 2007/11/02  Language: eng  Pages: 405-10

Authors:Ki, C. S., Park, S. Y., Kim, H. J., Jung, H. M., Woo, K. M., Lee, J. W., Park, Y. H.

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Abstract
We made a three-dimensional (3-D) nanofibrous fibroin scaffold (NFS) with high porosity (94%) and examined its feasibility in bone regeneration. Under scanning electron microscopy, MC3T3-E1 preosteoblasts on the scaffold showed more spread on the first day after seeding compared with a 2-D scaffold. MTT assay showed significantly increased proliferation in 3-D NFS compared with 2-D NFS 7 days after seeding (P < 0.05). Western immunoblotting for activated paxillin, FAK, AKT, C-Src, and ERK1/2 antibodies showed signals from the extracellular matrix were significantly increased in 3-D NFS. Newly developed 3-D electrospun NFS may be a good candidate for use in bone regeneration.