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Adipogenic Differentiation of Adipose-Derived Stem Cells on Collagen Microbeads
Periodical: Tissue Engineering and Regenerative Medicine ISBN: 1738-2696
Number: 4-11
Pages: 924-930
Authors:Kim, I., Park, H., Shin, Y., Kim, M.
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Abstract
Autologous fat transplantation for soft tissue augmentation has become a popular since its introduction in the late of 1800s. However, there are some limitations including unpredictable and continuous resorption after fat transplantation. Adipose derived stein cells(ASCs) are a promising cell source for adipose tissue engineering due to their plasticity and multilineage potential hi vitro and in vivo. Previously, we demonstrated that adipogenic differentiation of ASCs before transplantation could successfully enhance adipose tissue regeneration. Further, clinical trial showed effectiveness of autologous differentiated adipocytes therapy for treatment of soft tissue defects. Recent advances in tissue engineering have demonstrated the feasibility of using scaffold to overcome limitations of conventional 2-dimensional(2-D) culture system and to enhance therapeutic efficacy. Scaffold for adipose tissue engineering should be injectable and biodegradable as a cell delivery vehicle as well as provide a favorable surface for cell adhesion, proliferation and differentiation. In this study, we used collagen-based microbeads to assess the ability of human ASCs to proliferate and differentiate into adipocytes on microbeads. Human ASCs were seeded onto microbeads and differentiated with adipogenic condition. Cell growth, differentiation, function and in vivo adipose tissue formation were evaluated by fluorescence image analysis, Oil red 0 and Nile red staining, enzyme-linked immunosorbent assay and immunohistochemistry. ASCs attached and proliferated well on the microbeads and the number of cells increased more than 7 times for 8 days of culture. Cells grown on microbeads were differentiated into adipocytes with higher efficiency than 2-D Culture system based on differentiation rate and leptin secretion. When adipocytes differentiated on microbeads were injected into nude mouse subcutaneously microbeads were degraded over time and adipocytes formed new adipose tissue. This study
Autologous fat transplantation for soft tissue augmentation has become a popular since its introduction in the late of 1800s. However, there are some limitations including unpredictable and continuous resorption after fat transplantation. Adipose derived stein cells(ASCs) are a promising cell source for adipose tissue engineering due to their plasticity and multilineage potential hi vitro and in vivo. Previously, we demonstrated that adipogenic differentiation of ASCs before transplantation could successfully enhance adipose tissue regeneration. Further, clinical trial showed effectiveness of autologous differentiated adipocytes therapy for treatment of soft tissue defects. Recent advances in tissue engineering have demonstrated the feasibility of using scaffold to overcome limitations of conventional 2-dimensional(2-D) culture system and to enhance therapeutic efficacy. Scaffold for adipose tissue engineering should be injectable and biodegradable as a cell delivery vehicle as well as provide a favorable surface for cell adhesion, proliferation and differentiation. In this study, we used collagen-based microbeads to assess the ability of human ASCs to proliferate and differentiate into adipocytes on microbeads. Human ASCs were seeded onto microbeads and differentiated with adipogenic condition. Cell growth, differentiation, function and in vivo adipose tissue formation were evaluated by fluorescence image analysis, Oil red 0 and Nile red staining, enzyme-linked immunosorbent assay and immunohistochemistry. ASCs attached and proliferated well on the microbeads and the number of cells increased more than 7 times for 8 days of culture. Cells grown on microbeads were differentiated into adipocytes with higher efficiency than 2-D Culture system based on differentiation rate and leptin secretion. When adipocytes differentiated on microbeads were injected into nude mouse subcutaneously microbeads were degraded over time and adipocytes formed new adipose tissue. This study
Keywords
3 dimensional co-culture, CARTILAGE, CHONDROCYTES, chondrogenesis, COMPRESSION, DYNAMIC, EXPRESSION, INTERMITTENT HYDROSTATIC-PRESSURE, MARROW STROMAL CELLS, mechanical stimulation, Mesenchymal stem cells(MSCs), ORGANIZATION, PHENOTYPE, PROGENITOR CELLS, SOX9
3 dimensional co-culture, CARTILAGE, CHONDROCYTES, chondrogenesis, COMPRESSION, DYNAMIC, EXPRESSION, INTERMITTENT HYDROSTATIC-PRESSURE, MARROW STROMAL CELLS, mechanical stimulation, Mesenchymal stem cells(MSCs), ORGANIZATION, PHENOTYPE, PROGENITOR CELLS, SOX9
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search attributes
CellLine: Primary-hAdiposeSC
Morphology: Stem Cell
Origin: Adipose Tissue
Species: Human
Scaffold Form: bead/microsphereMorphology: Stem Cell
Origin: Adipose Tissue
Species: Human
Scaffold Material: Collagen