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A novel 3-D model for cell culture and tissue engineering
Periodical: Biomedical Microdevices ISBN: 1387-2176
Number: 4
Pages: 795-799
Authors:Zhang, X. L., Xie, Y. B., Koh, C. G., Lee, L. J.
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Abstract
A novel method of making microcapsules in a macrocapsule is demonstrated as a 3-D culture system in this article. Mouse embryonic stem (mES) cells as model cells were used in the 3-D culture space, and the cell viability and histological observation were conducted. Furthermore, Oct4 gene expression was evaluated for the undifferentiated status of mES cells in this 3-D model. The results showed that mES cells can grow in this 3-D model and retain their normal viability and morphology. This 3-D model allows mES cells to stay in the undifferentiated state better than 2-D culture systems. This work demonstrates a new 3-D tissue model which can provide an in vivo like microenvironment for non-differentiated mES cells with good immunoisolation. This approach may bridge the gap between traditional 2-D cell culture and animal models.
A novel method of making microcapsules in a macrocapsule is demonstrated as a 3-D culture system in this article. Mouse embryonic stem (mES) cells as model cells were used in the 3-D culture space, and the cell viability and histological observation were conducted. Furthermore, Oct4 gene expression was evaluated for the undifferentiated status of mES cells in this 3-D model. The results showed that mES cells can grow in this 3-D model and retain their normal viability and morphology. This 3-D model allows mES cells to stay in the undifferentiated state better than 2-D culture systems. This work demonstrates a new 3-D tissue model which can provide an in vivo like microenvironment for non-differentiated mES cells with good immunoisolation. This approach may bridge the gap between traditional 2-D cell culture and animal models.
Keywords
ADHESION, Cell orientation, CELLS, CONTACT GUIDANCE, DIFFERENTIATION, FABRICATION, Fibroblasts, FLY PHOTOPOLYMERIZATION, Focal contact, HUMAN-FIBROBLASTS, KERATINOCYTES, MICRO, MICROGROOVED POLYSTYRENE, PLGA fibers, Scaffold, SCAFFOLDS, WOUND CLOSURE
ADHESION, Cell orientation, CELLS, CONTACT GUIDANCE, DIFFERENTIATION, FABRICATION, Fibroblasts, FLY PHOTOPOLYMERIZATION, Focal contact, HUMAN-FIBROBLASTS, KERATINOCYTES, MICRO, MICROGROOVED POLYSTYRENE, PLGA fibers, Scaffold, SCAFFOLDS, WOUND CLOSURE
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CellLine: Primary-mouseEmbryoSC
Morphology: Stem Cell
Origin: Embryo
Species: Mouse
Scaffold Form: bead/microsphereMorphology: Stem Cell
Origin: Embryo
Species: Mouse
Scaffold Material: Alginate / sodium alginate