Back button

Human keratinocyte caspase-14 expression is altered in human epidermal 3D models by dexamethasone and by natural products used in cosmetics

Periodical: Arch Dermatol Res ISBN: 1432-069X (Electronic) 0340-3696 (Linking)  Number: 8  Date: 2013/04/23  Language: eng  Pages: 683-9

Authors:Kataoka, S., Hattori, K., Date, A., Tamura, H.
A copy of this paper may be available for free: Google Scholar Search Google Scholar
Caspase-14 is a cysteinyl-aspartate-specific proteinase that is specifically expressed in epidermal keratinocytes. Dysregulation of caspase-14 expression is implicated in impaired skin barrier formation. To elucidate the regulation of caspase-14 in differentiated keratinocytes, we characterized the expression of caspase-14 in normal human epidermal keratinocytes (NHEKs) and two types of three-dimensional (3D) human epidermis culture models, EPI-200 and EPI-201, via RT-PCR and immunoblot analyses. Caspase-14 expression was absent in subconfluent NHEKs, but was present in confluent NHEKs as well as those induced to differentiate by calcium. Caspase-14 expression levels in the 3D epidermis models were almost equal to that in the Ca(2+)-treated differentiated NHEKs. Despite the presence of caspase-14 expression in these models, caspase-14 activity was found only in the mature 3D skin model, EPI-200. This was confirmed by detection of a 17 kDa cleaved fragment of caspase-14 present only in the EPI-200 model. Since glucocorticoid (GC) receptor is required for skin barrier competence, we investigated whether the GC dexamethasone (Dex) and various natural components of common skin moisturizers affect caspase-14 expression in keratinocytes. Dex decreased caspase-14 expression in undifferentiated, but not differentiated, NHEKs. Conversely, Dex increased caspase-14 expression in both 3D skin models, although it did not alter caspase protease activity. Similar to treatment with Dex, treatment of the premature 3D skin mode, EPI-201 with a Galactomyces ferment filtrate markedly increased expression of caspase-14. Further, these results suggest that the effect of Dex, or lack thereof, on caspase-14 expression is dependent on the stage of keratinocyte differentiation.
Calcium Channels/metabolism, Cell Adhesion/drug effects, Cell Culture Techniques, Cell Differentiation/ drug effects, Cell Line, Tumor, Cell Movement/ drug effects, Cell Survival/drug effects, Cells, Cultured, Gene Expression/drug effects, Humans, Hypoglycemic Agents/pharmacology, Membrane Potentials/drug effects, Microtubule-Associated Proteins/genetics, Neoplasm Invasiveness, Neuroblastoma/genetics/metabolism/pathology, Peptides/ pharmacology, Protein Binding/drug effects, Receptors, Glucagon/genetics, Receptors, Urokinase Plasminogen Activator/genetics, Reverse Transcriptase Polymerase Chain Reaction, Synaptophysin/genetics, tau Proteins/genetics, Venoms/ pharmacology, Vitronectin/metabolism

3DCellculture.com's MAMI
search attributes

enhance this article