Back button

Low intensity pulse ultrasound stimulate chondrocytes growth in a 3-D alginate scaffold through improved porosity and permeability

Periodical: Ultrasonics ISBN: 0041-624x  Date: 2014/12/30  Language: eng  Pages: 43-52

Authors:Guo, G., Lu, L., Ji, H., Ma, Y., Dong, R., Tu, J., Guo, X., Qiu, Y., Wu, J., Zhang, D.
A copy of this paper may be available for free: Google Scholar Search Google Scholar
A 3-D scaffold culture system has been used to promote in producing functional chondrocytes for repairing damaged cartilage. In the present study, the low intensity pulse ultrasound (LIPUS) (P(-)=0, 0.055, 0.085 and 0.11MPa) was applied to improve the porosity and permeability of a 3-D alginate scaffold which was beneficial for the nutrition supply and metabolism during cell growth in 3-D alginate scaffold. The porosity and permeability of the scaffold was quantitatively analyzed based on scanning electron microscopy examination and fluorescence image observation. The results suggest that, for the scaffold exposed to LIPUS, its porosity and permeability could be significantly enhanced by the increasing LIPUS amplitude, which might be induced by the microstreaming shear stress generated by ultrasound-driven microbubble oscillations. Furthermore, the assessments of cell proliferation and collagen II expression confirmed that chondrocytes growth could be effectively promoted in 3-D alginate scaffolds treated by LIPUS, because of the improved scaffold porosity and permeability might benefit cell growth space and nutrition supply. It should also be noticed that appropriate LIPUS driving parameters should be adapted to achieve optimized chondrocytes culture effect in 3-D alginate scaffold.
*Cell Culture Techniques, *Chondrogenesis, *Hot Temperature, Aggrecans/metabolism, Antigens, Surface/metabolism, Chondrocytes/*cytology/metabolism, Collagen Type I/metabolism, Collagen Type II/metabolism, Glycosaminoglycans/metabolism, HSP72 Heat-Shock Proteins/metabolism, Humans, Immunohistochemistry, Immunophenotyping, Mesenchymal Stromal Cells/*cytology/metabolism

3DCellculture.com's MAMI
search attributes

enhance this article
CellLine: HeLa
  Morphology: Epithelial
  Origin: Cervix
  Species: Human
CellLine: Primary-rabbitArticularChondrocyte
  Morphology: Chondrocyte
  Origin: Joint
  Species: Rabbit
Scaffold Form: gel/hydrogel
Scaffold Material: Alginate / sodium alginate
Scaffold Origin: natural