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Cell response to single-walled carbon nanotubes in hybrid porous collagen sponges

Periodical: Colloids Surf B Biointerfaces ISBN: 0927-7765  Date: 2014/12/30  Language: eng  Pages: 63-9

Authors:Mao, H., Kawazoe, N., Chen, G.
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Three-dimensional (3D) porous collagen sponges incorporated with single-walled carbon nanotubes (SWCNTs) were prepared and used for 3D culture of bovine articular chondrocytes (BACs). The pore structures of the sponges were controlled by using ice particulates as a porogen material. The responses of cells to SWCNTs were investigated in this 3D cell culture system by evaluation of cell functions and cellular uptake of SWCNTs. The results showed that cells adhered and spatially distributed in the porous sponges. The incorporation of SWCNTs in the porous sponges promoted cell proliferation and production of sulfated glycosaminoglycans (sGAG). Confocal Raman imaging revealed that SWCNTs could be internalized by cells. The hybrid porous sponges not only provided nanostructured pore surfaces to facilitate cell proliferation and extracellular matrix (ECM) secretion but also supplied nanomaterials for cellular uptake which may be useful for biomedical applications.
3D cell culture, Caco-2 cells, Drug permeability assay, Pharmacokinetics, Tumor spheroids

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CellLine: Primary-cowarticularchondrocytes
  Morphology: Chondrocyte
  Origin: Joint
  Species: Cow
Scaffold Form: porous solid/sponge
Scaffold Material: Collagen
Scaffold Material: SWCNT/single-walled carbon nanotube
Scaffold Origin: synthetic