(Eng)   [ matched 1 times ] 

Abstract Cell-monolayer-based assays for chemotherapeutic drug discovery have proven to be highly artificial compared with physiological systems. The objective of this study was to culture cancer cells in a simple 3-dimensional (3D) collagen gel mode...

Periodical:  Assay Drug Dev Technol  2010/07/29

Authors:  Nirmalanandhan, V. S., Duren, A., Hendricks, P., Vielhauer, G., Sittampalam, G. S.

(Eng)   [ matched 1 times ] 

Stalled cell division in precursor bone cells and reduced osteoblast function are considered responsible for the microgravity-induced bone loss observed during spaceflight. However, underlying molecular mechanisms remains unraveled. Having overcome t...

Periodical:  J Cell Biochem  2010/07/27

Authors:  Monticone, M., Liu, Y., Pujic, N., Cancedda, R.

(Eng)   [ matched 1 times ] 

To establish an ex vivo expansion method of haematopoietic progenitor cells (HPCs) and erythroid cells, three-dimensional (3D) cultures of mouse bone marrow cells were performed, employing a porous polyvinyl formal (PVF) resin as a scaffold. In these...

Periodical:  J Tissue Eng Regen Med  2010/07/24

Authors:  Miyoshi, H., Murao, M., Ohshima, N., Tun, T.

(Eng)   [ matched 1 times ] 

Scaffold-free three-dimensional (3D) cultures provide clinical potential in cartilage regeneration. The purpose of this study was to characterize a scaffold-free 3D membrane-based culture system, in which human articular chondrocytes were cultivated ...

Periodical:  J Tissue Eng Regen Med  2010/07/24

Authors:  Mayer-Wagner, S., Schiergens, T. S., Sievers, B., Redeker, J. I., Schmitt, B., Buettner, A., Jansson, V., Mueller, P. E.

(eng)   [ matched 1 times ] 

Drug discovery programmes require accurate in vitro systems for drug screening and testing. Traditional cell culture makes use of 2D (two-dimensional) surfaces for ex vivo cell growth. In such environments, cells are forced to adopt unnatural charact...

Periodical:  Biochem Soc Trans  2010/07/28

Authors:  Maltman, D. J., Przyborski, S. A.

(eng)   [ matched 1 times ] 

Purpose: The purpose of this study was to determine whether osseous tissues engineered in three-dimensional (3D) environments preserved their mineralizing capacity and retained biologic characteristics when cultured on dental implant surfaces. Materi...

Periodical:  Int J Oral Maxillofac Implants  2010/07/27

Authors:  Ko, Y. J., Zaharias, R. S., Seabold, D. A., Lafoon, J. E., Schneider, G. B.

(Eng)   [ matched 1 times ] 

Intervertebral disc degeneration is considered a major source of low back pain. We therefore examined an absorbable polyglycolic acid (PGA) biomaterial for its utility to support disc tissue regeneration. Microdiscectomy for lumbar disc herniation wa...

Periodical:  J Tissue Eng Regen Med  2010/07/28

Authors:  Hegewald, A. A., Enz, A., Endres, M., Sittinger, M., Woiciechowsky, C., Thome, C., Kaps, C.

(Eng)   [ matched 1 times ] 

BACKGROUND CONTEXT: Understanding gene expression patterns of disc cells in culture is important as we develop biologic therapies for disc degeneration. The objective of the present study was to determine if cells from more degenerated discs expresse...

Periodical:  Spine J  2010/07/24

Authors:  Gruber, H. E., Hoelscher, G. L., Hanley, E. N., Jr.

(Eng)   [ matched 1 times ] 

In the present in vitro study, the axon growth promoting effects of human neural progenitor-derived astrocytes (hNP-AC) were investigated in simple 2D- as well as in more complex 3D-culture systems. The interactions of the hNP-AC with migrating Schwa...

Periodical:  Biomaterials  2010/07/27

Authors:  Fuhrmann, T., Hillen, L. M., Montzka, K., Woltje, M., Brook, G. A.

Keywords:  Animals, Bioreactors, Cell Adhesion/physiology, Cell Aggregation, Cell Culture Techniques/ instrumentation, Cells, Cultured, Dimethylpolysiloxanes/ chemistry, Hepatocytes/ cytology/ physiology, Male, Membranes, Artificial, Oxygen/ chemistry, Permeability, Rats, Rats, Wistar

(Eng)   [ matched 1 times ] 

BACKGROUND: For current tissue engineering or regenerative medicine strategies, chondrocyte- or mesenchymal stem cell (MSC)-seeded constructs are typically cultured in normoxic conditions (20% oxygen). However, within the knee joint capsule a lower o...

Periodical:  Osteoarthritis and Cartilage  2010/07/24

Authors:  Buckley, C. T., Vinardell, T., Kelly, D. J.

(eng)   [ matched 1 times ] 

A cell`s microenvironment plays a primary role in defining cell fate during tissue development, physiological function, and pathological dysfunction. Understanding the key components and interactions within these microenvironments is critical for eff...

Periodical:  Curr Stem Cell Res Ther  2010/03/11

Authors:  Levengood, S. L., Murphy, W. L.

Three-dimensional (3D) cellular assays closely mimic the in vivo milieu, providing a rapid, inexpensive system for screening drug candidates for toxicity or efficacy in the early stages of drug discovery. However, 3D culture systems may suffer from m...

Periodical:  Acs Nano

Authors:  Zhang, Haiyuan, Lee, Moo-Yeal, Hogg, Michael G., Dordick, Jonathan S., Sharfstein, Susan T.

The promising potential of a RAD-16 self-assembly-peptide hydrogel as a scaffold for tissue-engineered cartilage was investigated. Within 3 weeks of in vitro culture, chondrocytes within the hydrogel produced a high amount of GAG and type-II collagen...

Periodical:  Macromolecular Bioscience

Authors:  Jingping Liu, Hong Song, Lanlan Zhang, Hongyan Xu, Xiaojun Zhao

The development of novel breast cancer therapies depends on appropriate experimental models of disease pathogenesis. In recent years, there has been a proliferation of sophisticated models for breast cancer research. We describe here novel models tha...

Periodical:  Drug Discovery Today: Disease Models

Authors:  Gupta, Piyush B., Kuperwasser, Charlotte

(eng)   [ matched 1 times ] 

Ocular angiogenesis is the leading cause of blindness and is associated with diabetic retinopathy and age-related macular degeneration. We describe, in this report, our preliminary studies using a horizontally rotating bioreactor (HRB), developed by ...

Periodical:  Tissue Eng  2003/11/25

Authors:  Dutt, K., Sanford, G., Harris-Hooker, S., Brako, L., Kumar, R., Sroufe, A., Melhado, C.