(Eng)   [ matched 1 times ] 

The microenvironment plays a significant role in human cancer progression. However, the role of the tumor microenvironment in the epigenetic control of genes critical to cancer progression remains unclear. As transient E-cadherin expression is centra...

Periodical:  Epigenetics  2011/12/31

Authors:  Desrochers, T. M., Shamis, Y., Alt-Holland, A., Kudo, Y., Takata, T., Wang, G., Jackson-Grusby, L., Garlick, J. A.

(eng)   [ matched 1 times ] 

A three-dimensional culture system supports the development of primate preantral follicles to the antral stage with appreciable steroid production. This study assessed i) whether in vitro developmental competence of follicles is age dependent, ii) th...

Periodical:  Reproduction  2010/08/24

Authors:  Xu, J., Bernuci, M. P., Lawson, M. S., Yeoman, R. R., Fisher, T. E., Zelinski, M. B., Stouffer, R. L.

(chi)   [ matched 1 times ] 

For the purpose of fetal membrane healing, amnion mesenchymal cells were embedded in three-dimensional (3D) collagen I, and amnion epithelial cells were placed on top of it to build the amniotic cells/collagen I matrix complex to simulate the archite...

Periodical:  Sheng Wu Yi Xue Gong Cheng Xue Za Zhi  2010/05/21

Authors:  Liu, F., Qi, H.

(Eng)   [ matched 1 times ] 

Recently, in situ tissue engineering has emerged as a new approach to obtain autologous, living replacement tissues with off-the-shelf availability. The method is based on the use of an instructive biodegradable scaffold that is capable of repopulati...

Periodical:  Tissue Eng Part C Methods  2012/01/10

Authors:  Smits, A., Driessen-Mol, A., Bouten, C., Baaijens, F.

Keywords:  Animals, Capsules, Cell Culture Techniques, Collagen/ pharmacology, Drug Combinations, Fetus/cytology, Immunohistochemistry, Laminin/ pharmacology, Mice, Mice, Inbred BALB C, Proteoglycans/ pharmacology, Pulmonary Alveoli/cytology/embryology/ physiology/ultrastructure, Reverse Transcriptase Polymerase Chain Reaction, Tissue Engineering/ methods, Tissue Scaffolds/ chemistry

(eng)   [ matched 1 times ] 

This study attempted to use collagen-Matrigel as extracellular matrix (ECM) to supply cells with three-dimensional (3D) culture condition and employ alginate-poly-l-lysine-alginate (APA) microcapsules to control the formation of alveolus-like structu...

Periodical:  J Cell Mol Med  2010/10/30

Authors:  Zhang, W. J., Lin, Q. X., Zhang, Y., Liu, C. T., Qiu, L. Y., Wang, H. B., Wang, Y. M., Duan, C. M., Liu, Z. Q., Zhou, J., Wang, C. Y.

(Eng)   [ matched 1 times ] 

Inverse opal scaffolds have recently emerged as a novel class of scaffolds with uniform and controllable pore sizes for tissue engineering to provide better nutrient transport, a uniform cell distribution, and an adjustable microenvironment for cell ...

Periodical:  Macromol Rapid Commun  2012/01/11

Authors:  Zhang, Y., Choi, S. W., Xia, Y.

(Eng)   [ matched 1 times ] 

Fabrication of 3-D highly porous, bioactive, and mechanically competent scaffolds represents a significant challenge of bone tissue engineering. In this work, Bioglass(R)-derived glass-ceramic scaffolds actually fulfilling this complex set of require...

Periodical:  J Biomater Appl  2011/12/31

Authors:  Baino, F., Ferraris, M., Bretcanu, O., Verne, E., Vitale-Brovarone, C.

(Eng)   [ matched 1 times ] 

BackgroundRecent studies identified mesenchymal stem cells (MSC) as major players in vascular remodelling and the sub-endothelial compartment as the stem cell niche. The uraemic microenvironment predisposes to increased levels of reactive oxygen spec...

Periodical:  Nephrol Dial Transplant  2011/12/31

Authors:  Kramann, R., Couson, S. K., Neuss, S., Floege, J., Knuchel, R., Schneider, R. K.

(Eng)   [ matched 1 times ] 

We report the adaptation of dendrimer-based nonviral expression system for ciliary neurotrophic factor (CNTF) overproduction in human mesenchymal stem cells (hMSCs) embedded into fibrin-based three-dimensional (3D) matrix. Time-restricted neurotrophi...

Periodical:  J Pharm Sci  2012/01/04

Authors:  Shakhbazau, A., Shcharbin, D., Petyovka, N., Goncharova, N., Seviaryn, I., Kosmacheva, S., Bryszewska, M., Potapnev, M.

(Eng)   [ matched 1 times ] 

OBJECTIVE: To examine the impact of hormones used for controlled ovarian hyperstimulation (COH) on normal and malignant breast cell growth and proliferation. DESIGN: In vitro study of cultured normal and malignant breast cell lines. SETTING: Academic...

Periodical:  Fertil Steril  2011/12/23

Authors:  Boukaidi, S. A., Cooley, A., Hardy, A., Matthews, L., Zelivianski, S., Jeruss, J. S.

(Eng)   [ matched 1 times ] 

The focus on creating tissue engineered constructs of clinically relevant sizes requires new approaches for monitoring construct health during tissue development. A few key requirements are that the technology be in situ, non-invasive, and provide te...

Periodical:  Biomaterials  2011/12/24

Authors:  Dunkers, J. P., Lee, Y. J., Chatterjee, K.

(eng)   [ matched 1 times ] 

Cells in a functional tissue display a highly interactive relation with their neighboring cells and associated biochemical milieu. Serious disruption in the existing homeostatic balance in the extra-cellular matrix (ECM) may lead to abnormal response...

Periodical:  Front Biosci (Elite Ed)  2011/12/29

Authors:  Dutta, R. C., Dutta, A. K.

Keywords:  Animals, Cattle, Cell Shape/drug effects, Coculture Techniques, Fibrillar Collagens/ pharmacology, Fibroblasts/cytology/drug effects/ultrastructure, Human Umbilical Vein Endothelial Cells/cytology/drug effects/ultrastructure, Humans, Hyaluronic Acid/analogs & derivatives/ pharmacology, Hydrogel/ pharmacology, Intercellular Signaling Peptides and Proteins/ metabolism, Neovascularization, Physiologic/ drug effects, Polyesters/chemistry/ pharmacology, Tissue Scaffolds/ chemistry

(eng)   [ matched 1 times ] 

A significant stumbling block in the creation of functional three-dimensional (3D) engineered tissues is the proper vascularization of the constructs. Furthermore, in the context of electrospinning, the development of 3D constructs using this techniq...

Periodical:  Biomaterials  2011/08/03

Authors:  Ekaputra, A. K., Prestwich, G. D., Cool, S. M., Hutmacher, D. W.

(Eng)   [ matched 1 times ] 

Realizing the potential of human pluripotent stem cell (hPSC)-based therapy requires the development of defined scalable culture systems with efficient expansion, differentiation and isolation protocols. We report an engineered 3D microfiber system t...

Periodical:  Biomaterials  2011/12/27

Authors:  Lu, H. F., Narayanan, K., Lim, S. X., Gao, S., Leong, M. F., Wan, A. C.

(eng)   [ matched 1 times ] 

Three-dimensional (3D) engineered tissue constructs are a novel and promising approach to tissue repair and regeneration. 3D tissue constructs have the ability to restore form and function to damaged soft tissue unlike previous methods, such as plast...

Periodical:  J Biomed Biotechnol  2011/12/29

Authors:  Ma, J., Holden, K., Zhu, J., Pan, H., Li, Y.